Abstract: This study analyzes the effect of culture on entrepreneurship in Sub-Saharan Africa (SSA) using a panel of 45 countries over the period 1991 to 2018. Using the two stage GMM, results show that (a) British legal origins negatively relate to entrepreneurship unlike the French legal origin; (b) Muslim and Protestant religions positively influence entrepreneurship whilst the Catholic and other religions rather negatively associate to entrepreneurial activity in SSA; (c) the number of official languages spoken in a country negatively affect entrepreneurship and (d) ethnic fragmentation has no impact on entrepreneurship in SSA. The study thereon recommends that entrepreneurship education should be put in instated from basic levels of education with the aim of mitigating the negative effect of some religious orientations on entrepreneurship.